Physicochemical Characterization of Native and Asialo Human Chorionic Gonadotropin
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چکیده
منابع مشابه
Physicochemical characterization of native and asialo human chorionic gonadotropin.
Human chorionic gonadotropin (HCG) was desialized by treatment with neuraminidase to yield asialo-HCG, and comparative physicochemical studies were carried out on HCG and asialo-HCG. The E:trn value at 276 nm was 5.47 for HCG and 5.72 for asialo-HCG. Intrinsic viscosities of HCG and asialo-HCG at near isoionic point were estimated to be 3.4 and 3.2 ml per g, respectively. HCG had an isoionic po...
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hCG is a putative thyroid stimulator. The present studies were undertaken to examine its interaction and that of its desialylated variant asialo-hCG with recombinant human TSH (hTSH) receptor (hTSHr). To this end, we transfected a human thyroid carcinoma cell line (HTC) lacking endogenous TSHr with the full-length cDNA of the hTSHr. Unlike the wild type, the transfected cells, termed HTC-TSHr c...
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A highly active preparation of human chorionic gonadotropin has been obtained by simple methods of column chromatography on diethylaminoethyl Sephadex and Sephadex G-100. The preparation appeared homogeneous by disc and immunoelectrophoresis, ultracentrifugation, and end group analysis. It assayed at 12,000 i.u. per mg by the rat prostrate gland and accessory sex organ method. The ~20,~ value w...
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Angiogenesis and vascular remodeling are crucial processes in tumor invasion and metastasis as well as in embryo implantation and normal development of the placenta. We have previously shown that hCG expressed in trophoblast and various malignant tumors promotes cellular motility and that uterine endothelium expresses hCG/LH receptor in vivo. In this study hCG was proposed to promote angiogenes...
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Background and Aims: Some nanoparticles can be used in immunoassays to increase sensitivity. This study aimed to evaluate a novel nano-immunoassay based on bovine serum albumin nanoparticles (BSA NPs). Materials and methods: At first, the nanostructure was synthesized, and then applied as a tag in the nano-immunoassay. Then the concentration of β-subunit of human chorionic gonadotropin ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1971
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)45876-6